The reason to calibrate is to get the most accurate measurement of your sample.Įven if both of the microscopes used on the same sample were exactly the same but were not calibrated, a variation in magnification factors can lead to a mixture of results, one of which may not be accurate and can lead to incorrect data being recorded and used later.Ĭompanies such as laboratories or hospitals may hire specialists to do their calibration for them but it’s still wise for the supervisor to know how calibration works, so they can ensure the specialists are doing a good job.Īs students and hobbyists, you’ll need to know how to calibrate your own microscope to ensure the information you obtain is correct.Ĭalibration is performed using an eyepiece graticule (or reticle) and a stage micrometer. Using a microscope that’s calibrated means that the same results will be produced on the exact same sample under the same conditions if you were to use an entirely different microscope that was also calibrated. Repeat the process for your other objective lenses.Calculate the value of the ocular units in micrometers.
Align the eyepiece graticule and stage micrometer.Set up the eyepiece graticule and stage micrometer.
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